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GrainGenes Probe Report: WMC289

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC289  [ Marker Report ]
Locus	Xwmc289 [ Show all 13 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13d2 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 164 bases located on 5D, 198 bases located on 5B Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)13 221 to 246, (CT)10 178 to 197.
Type	PCR SSR
PCR primers	Forward cATATgcATgcTATgcTggcTA at 146 length 22 bases, Tm 63 Reverse AgccTTTcAAATccATccAcTg at 324 length 22 bases, Tm 64
SSR size	200 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC289
Source Species	Triticum aestivum