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GrainGenes Probe Report: WMC286

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC286  [ Marker Report ]
Locus	Xwmc286-7A Xwmc286.5 Xwmc286.2 Xwmc286.4
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f1.2 Donor: Jager-Gussen, Saaten Union Trimmed to insert at both ends. Multiple inserts were joined 371-380 by single linker - this is second insert. (CA)9 328 to 345, (GA)14 346 to 373. Weak homology with 1b11.
Type	PCR SSR
PCR primers	Forward cgggTTcTcTggATcggTTc at 211 length 20 bases, Tm 66 Reverse ccccggccTTccTcATTcTT at 373 length 20 bases, Tm 68
SSR size	182 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide (several loci).
Sequence	WMC286
Source Species	Triticum aestivum