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GrainGenes Probe Report: WMC285

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC285  [ Marker Report ]
Locus	Xwmc285 Xwmc285-4D wmc285
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 13f1.1 Donor: Jager-Gussen, Saaten Union Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 298 bases located on 4D Trimmed to insert at 3' end, 5' end not located. Multiple inserts (2?) joined 371-380 by single linker - this is first insert . (CA)10 18 to 37, (CA)20 73 to 112.
Type	PCR SSR
PCR primers	Forward TgTggTTgTATTTgcggTATgg at 42 length 22 bases, Tm 64 Reverse TTgTggTgcTgAgTTAgcTTgT at 309 length 22 bases, Tm 62
SSR size	289 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC285
Source Species	Triticum aestivum