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GrainGenes Probe Report: WMC281

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC281
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3b12 Donor: Keller, Zurich No trimming done to sequence. (CA)20 48 to 87, (CA)8 90 to 105,
Type	PCR SSR
PCR primers	Forward AcccAgccTcTgcATATTTA at 2 length 20 bases, Tm 59 Reverse TTTcATcTTccAcAggAcAA at 244 length 20 bases, Tm 59
SSR size	262 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC281
Source Species	Triticum aestivum