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GrainGenes Probe Report: WMC279

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC279
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3a11 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 222 bases located on 7B No trimming done to sequence. Multple inserts (2) joined 125-142 by linkers. First insert is a duplicate of 7G6. (GT)15 1 to 30, (GCG)6(GTG)(GAG) at 206-230. Primers are for second insert.
Type	PCR SSR
PCR primers	Forward cTcTgAcTcTgAcATcATgggc at 147 length 22 bases, Tm 64 Reverse gcAgAgcTgAccgTgcATTAcT at 347 length 22 bases, Tm 66
SSR size	222 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC279
Source Species	Triticum aestivum