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GrainGenes Probe Report: WMC276

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC276  [ Marker Report ]
Locus	Xwmc276-7B [ Show all 11 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e9.2 Donor: Keller, Zurich Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 283-306 bases located on 7B Second insert in 3e9. (CA)19 203 to 240, (CA)13 973 to 998, (CA)9 1000 to 1017, (CA)23 1026 to 1071. Primers shown are for second insert (coordinates refer to total insert).
Type	PCR SSR
PCR primers	Forward gAcATgTgcAccAgAATAgc at 817 length 20 bases, Tm 59 Reverse AgAAgAAcTATTcgAcTccT at 1089 length 20 bases, Tm 52
SSR size	292 bases expected, Chinese Spring
Amplification Conditions	51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC276
Source Species	Triticum aestivum