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GrainGenes Probe Report: WMC275

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC275
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e9.1 Donor: Keller, Zurich No trimming done to sequence. Multiple inserts (2) joined 636-653 by linkers.(CA)19 203 to 240, (CA)13 973 to 998, (CA)9 1000 to 1017, (CA)23 1026 to 1071. Primers shown are for first insert. Bases 1027 to 1134 show homology to 25f12 bases 96 to 203 (complement) (108 in longest run, maxScore = 44).
Type	PCR SSR
PCR primers	Forward TTgccTTTgAAATgTATggcTAgTAT at 142 length 26 bases, Tm 63 Reverse TTTgAgcAcTTTTAgcAcTgAAATcT at 330 length 26 bases, Tm 63
SSR size	214 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC275
Source Species	Triticum aestivum