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GrainGenes Probe Report: WMC271

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC271
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3c8 Donor: Keller, Zurich No trimming done to sequence. (GT)10 372 to 391, (GT)23 398 to 443.
Type	PCR SSR
PCR primers	Forward AgcggATccTTgTgcAcTTcgg at 236 length 22 bases, Tm 71 Reverse TcAggccTggcTAgcAATcTcA at 506 length 22 bases, Tm 68
SSR size	292 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC271
Source Species	Triticum aestivum