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GrainGenes Probe Report: WMC270

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC270
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3b8 Donor: Keller, Zurich No trimming done to sequence. (CA)14 188 to 215. Software struggled to find these primers.
Type	PCR SSR
PCR primers	Forward cAAgggccATTgTgTTgTcT at 12 length 20 bases, Tm 63 Reverse cAAcccAAgccTAccTgTgT at 211 length 20 bases, Tm 62
SSR size	219 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC270
Source Species	Triticum aestivum