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GrainGenes Probe Report: WMC267

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC267  [ Marker Report ]
Locus	Xwmc267
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e7.1 Donor: Keller, Zurich No trimming done to sequence. Multple inserts (3) joined 316-333, and at 636-654 by linkers. (CA)14 413 to 440, (CA)8 785 to 800, (GT)25 157 to 206, (CT)8 769 to 784. Third insert is duplicate of 3d7. Primers shown are for first insert.
Type	PCR SSR
PCR primers	Forward TcTTcAccccATAATTggAgAAgccT at 28 length 26 bases, Tm 69 Reverse TgcTTATTcTgcgcAcTggATgccTA at 218 length 26 bases, Tm 73
SSR size	216 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC267
Source Species	Triticum aestivum