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GrainGenes Probe Report: WMC266

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC266
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3d7 Donor: Keller, Zurich No trimming done to sequence. Multiple inserts (2) joined 272-290 by linkers. First insert is member of 1g1 family. (CA)15 429 to 458, (GA)15 212 to 241, (CT)12 405 to 428. Bases 516 to 568 show homology to 10a3 bases 72 to 124 (complement) (53 in longest run, maxScore = 125), duplicate, 3d7 has priority.
Type	PCR SSR
PCR primers	Forward ATgTATTTAcgAgcATcgAccg at 377 length 22 bases, Tm 63 Reverse ATggTTAcTcAgcccAcATTcA at 604 length 22 bases, Tm 63
SSR size	249 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC266
Source Species	Triticum aestivum