GrainGenes Probe Report: WMC265
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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
Probe
WMC265 [ Marker Report ]
Locus
Xwmc265-2B Xwmc265 wmc265 wmc265-2B wmc265-6B
Reference
Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4d6 Donor: Gupta, Meerut Trimmed to insert at 3' end. (GT)18 151 to 186. Bases 47-167 (ie. 5' side of microsatellite) match part of 1B3.
Type
PCR SSR
PCR primers
Forward gTggATAAcATcATggTcAAc at 15 length 21 bases, Tm 57
Reverse TAcTTcgcAcTAgATgAgccT at 257 length 22 bases, Tm 59
SSR size
263 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence
WMC265
Source Species
Triticum aestivum
GrainGenes Probe Report: WMC265
[Submit comment/correction][What is a probe?]
Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.
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