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GrainGenes Probe Report: WMC263

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC263
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b6 Donor: Gupta, Meerut Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 313 bases located on 3A Trimmed to insert at 3' end. (GGC)6 171 to 188.
Type	PCR SSR
PCR primers	Forward ggAggAgATTTAgTgccTgAcc at 56 length 22 bases, Tm 63 Reverse ATcAgAcggccgAAAgTTAgAc at 348 length 22 bases, Tm 63
SSR size	314 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC263
Source Species	Triticum aestivum