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GrainGenes Probe Report: WMC260

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC260
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 4e4
Donor: Gupta, Meerut
Trimmed to insert at 5' end and to Rsa1 at 3' end. (GA)26 42 to 93.
Type
PCR
SSR
PCR primers
Forward cAAgATcgTgccAAATcAAg at 5 length 20 bases, Tm 61
Reverse gTAccAgTAgcTATgTgTTAgatctc at 90 length 26 bases, Tm 57
SSR size
111 bases expected, Chinese Spring
Amplification Conditions
tested 51C anneal, no product;
Sequence
WMC260
Source Species
Triticum aestivum