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GrainGenes Probe Report: WMC258

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC258  [ Marker Report ]
Locus	Xwmc258-4A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4f3 Donor: Gupta, Meerut Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 275 bases located on 4A No trimming done to sequence. (GT)26 115 to 166. Bases 251 to 270 show homology to 17c8 bases 101 to 120 (20 in longest run, maxScore = 43). Bases 112 to 166 show homology to 20b7 bases 241 to 295 (55 in longest run, maxScore = 61).
Type	PCR SSR
PCR primers	Forward gcgATgTcAgATATccgAAAgg at 75 length 22 bases, Tm 65 Reverse AccAggAcAccAgAAcAgcAAT at 346 length 22 bases, Tm 65
SSR size	293 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC258
Source Species	Triticum aestivum