Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC256

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC256  [ Marker Report ]
Locus	Xwmc256-6A Xwmc256 wmc256-6A wmc256
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4h2.1 Donor: Gupta, Meerut Small linker like sequence at 234-241 in original ie. multiple inserts (2) in this sequence, this record shows first insert only (up to but excluding linker). No trimming done to sequence at 5'. (CA)12 65 to 88.
Type	PCR SSR
PCR primers	Forward ccAAATcTTcgAAcAAgAAccc at 37 length 22 bases, Tm 64 Reverse AccgATcgATggTgTATAcTgA at 132 length 22 bases, Tm 61
SSR size	117 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC256
Source Species	Triticum aestivum