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GrainGenes Probe Report: WMC255

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC255
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4g2 Donor: Gupta, Meerut Trimmed to insert at 3' end. Sequence possibly starts with EcoR1 at 5' end. (GT)4(TT)(GT)7(AT)(GT)5 at 24-59. Very difficult to find reverse primer with high enough Tm, forward primer may overlap linker.
Type	PCR SSR
PCR primers	Forward TcgAggcgcgTggATAAc at 6 length 18 bases, Tm 65 Reverse cAccTTgcATATATgAcTgAgc at 64 length 22 bases, Tm 60
SSR size	76 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC255
Source Species	Triticum aestivum