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GrainGenes Probe Report: WMC254

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC254  [ Marker Report ]
Locus	Xwmc254-1A [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b2 Donor: Gupta, Meerut No trimming done to sequence. (CA)28 31 to 86. Forward primer is not ideal.
Type	PCR SSR
PCR primers	Forward AgTAATcTggTccTcTcTTcTTcT at 2 length 24 bases, Tm 58 Reverse AggTAATcTccgAgTgcAcTTcAT at 171 length 24 bases, Tm 64
SSR size	193 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC254
Source Species	Triticum aestivum