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GrainGenes Probe Report: WMC253

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC253
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4h1 Donor: Gupta, Meerut No trimming done to sequence. (CA)13 63 to 88. Bases 163-232 match part of 11c4. Reverse primer avoids this. Bases 158 onwards were vector sequence (removed 18.3.98).
Type	PCR SSR
PCR primers	Forward TcAccgATTgTAAATAgcAccA at 10 length 22 bases, Tm 62 Reverse gATAAATcAcTTcgggTgAggg at 126 length 22 bases, Tm 63
SSR size	138 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC253
Source Species	Triticum aestivum