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GrainGenes Probe Report: WMC251

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC251
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4b1 Donor: Gupta, Meerut No trimming done to sequence. (GT)17 39 to 72. Struggled to get these primers, use at close to Tm (may get mispriming at low temperature).
Type	PCR SSR
PCR primers	Forward gAAgAgTgAgTgTgcAAAAg at 18 length 20 bases, Tm 55 Reverse cgAgAgTgATggcgTAgATT at 140 length 20 bases, Tm 60
SSR size	142 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC251
Source Species	Triticum aestivum