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GrainGenes Probe Report: WMC250

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC250
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4A1 Donor: Gupta, Meerut No trimming done to sequence. (GA)18 121 to 156. Bases 1-148 (complement) (ie 5' side of microsatellite) match part of 4H4 . Forward primer contains single base insertion difference with respect to 5H4.
Type	PCR SSR
PCR primers	Forward ATgcTTATggAccgTgAcAgAg at 91 length 22 bases, Tm 63 Reverse AgTgcAAAggcgccgATTTATg at 182 length 22 bases, Tm 69
SSR size	113 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC250
Source Species	Triticum aestivum