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GrainGenes Probe Report: WMC248

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC248
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d5.3 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 231 bases located on 2D Third insert only shown here (without linkers). Bases 1-319 ( complement) match part of 7a3 (3). (CA)19 228 to 265. Primers shown are for third insert, the forward primer is within the homologous region to 7A3(3) but has 2 internal bases different. To exploit this must use anneal at close to Tm (try 61 first).
Type	PCR SSR
PCR primers	Forward gTATgcATgcTcAgTgTgcTAT at 82 length 22 bases, Tm 60 Reverse TTAAAAgcTgTcAcgAgcgAAg at 304 length 22 bases, Tm 63
SSR size	243 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC248
Source Species	Triticum aestivum