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GrainGenes Probe Report: WMC247

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC247  [ Marker Report ]
Locus	Xwmc247-5B Xwmc247
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5d5.2 Donor: Sharp, Camden Derived from full sequence - this is the second insert only, without linkers. Primers shown are for this insert insert. (GT)13 107-132.
Type	PCR SSR
PCR primers	Forward cATgTTgATAAccgAcTcgcAg at 80 length 22 bases, Tm 65 Reverse TgAAAcTAggggccAcgAATAA at 137 length 22 bases, Tm 64
SSR size	79 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC247
Source Species	Triticum aestivum