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GrainGenes Probe Report: WMC245

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC245  [ Marker Report ]
Locus
Xwmc245-2B
[ Show all 8 ]
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5b5
Donor: Sharp, Camden
Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 129 bases located on 2A, 150 bases located on 2D
Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts in this clone (2) separated at 314-331 by linkers. First insert is duplicate of 7a11. (CA)10 261 to 280, (CA)10 291 to 310, (CA)13 496 to 521, (CT)8 480 to 495. Bases 136 to 167 show homology to 2g3 bases 76 to 107 (32 in longest run, maxScore = 79). Bases 3 to 19 show homology to 25d1 bases 14 to 30 (17 in longest run, maxScore = 35). True duplicate with 2g3, 5b5 has priority.
Type
PCR
SSR
PCR primers
Forward gcTcAgATcATccAccAAcTTc at 436 length 22 bases, Tm 63
Reverse AgATgcTcTgggAgAgTccTTA at 564 length 22 bases, Tm 61
SSR size
150 bases expected, Chinese Spring
Amplification Conditions
61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence
WMC245
Source Species
Triticum aestivum