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GrainGenes Probe Report: WMC244

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC244
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5h4 Donor: Sharp, Camden Trimmed to insert at 5' end and to Rsa1 at 3' end. Bases 1- 159 (complement) match part of 1h6, 3d1. (CT)16 326 to 357. Forward primer avoids common sequence with 1H6. Duplicate 8G1. Reverse primer has 3 G deletions relative to 4A1. Region 150-250 contains partially duplicated sequence. Bases 326-474 match part of 4A1 (complement).
Type	PCR SSR
PCR primers	Forward TcTccAcTTTTATcgTgTgTTg at 266 length 22 bases, Tm 60 Reverse ggTTTgAcTTTTcAgAAcATcc at 390 length 22 bases, Tm 59
SSR size	146 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC244
Source Species	Triticum aestivum