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GrainGenes Probe Report: WMC243

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC243  [ Marker Report ]
Locus	Xwmc243a-2B [ Show all 12 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5e4 Donor: Sharp, Camden Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 158 bases located on 7D, 165 bases located on 2B Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)15 39 to 68. Small common sequence with 11D6 avoided.
Type	PCR SSR
PCR primers	Forward cgTcATTTccTcAAAcAcAccT at 8 length 22 bases, Tm 63 Reverse AccggcAgATgTTgAcAATAgT at 163 length 22 bases, Tm 62
SSR size	177 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC243
Source Species	Triticum aestivum