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GrainGenes Probe Report: WMC242

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Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.


Probe
WMC242
Reference
ReferenceSomers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks
Wheat Microsatellite Consortium Project 1, Plate reference 5a4
Donor: Sharp, Camden
Trimmed to insert at 5' end and to Rsa1 at 3' end. (CA)10 110 to 129. Bases 189 to 226 show homology to 17d11 bases 14 to 51 (complement) (38 in longest run, maxScore = 44). Bases 178 to 226 show homology to 10d10 bases 233 to 281 (complement) (49 in longest run, maxScore = 118). Bases 30 to 99 show homology to 24f4 bases 66 to 135 (complement) (70 in longest run, maxScore = 91).
Type
PCR
SSR
PCR primers
Forward ggTATcccTgcTAAgTTTTTcAATg at 19 length 25 bases, Tm 63
Reverse cTAATATTTAggAAcAgAAggAgTA at 217 length 25 bases, Tm 54
SSR size
223 bases expected, Chinese Spring
Amplification Conditions
51C anneal, product;
Sequence
WMC242
Source Species
Triticum aestivum