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GrainGenes Probe Report: WMC240

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC240
Locus	Xwmc240
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5g3 Donor: Sharp, Camden Trimmed to Rsa1 at 5' end, and to insert at 3' end. Bases 340 -441 (complement)ie. 5' side of microsatellite match part of 9d1. Bases 2-176 match part of 4A6 (complement). (CA)11 412 to 433. Forward primer is outside of regions that match other sequences. Bases 405 to 433 show homology to 10A1.2 bases 35 to 63 (complement) (29 in longest run, maxScore = 42), true duplicate with 10a1.2, 5g3 has priority. Bases 204 to 233 show homology to 25c10 bases 34 to 63 (30 in longest run, maxScore = 60). Duplicate with 25c10, 5g3 has priority. Bases 330 to 379 show homology to 22e4 bases 62 to 111 (complement) (50 in longest run, maxScore = 265). Duplicate with 22e4, 5g3 has priority.
Type	PCR SSR
PCR primers	Forward TAAAcgAgAggATgcAccgAcT at 303 length 22 bases, Tm 65 Reverse cAcgTTAgAAggTgcAAcAggA at 454 length 22 bases, Tm 64
SSR size	173 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC240
Source Species	Triticum aestivum