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GrainGenes Probe Report: WMC239

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC239
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5f3 Donor: Sharp, Camden Trimmed to Rsa1 at 5' end and to insert at 3' end.(GT)13 43 to 68. Forward primer Tm is estimation after adding 3 bases to 5' end.
Type	PCR SSR
PCR primers	Forward GTAccAAgAcTTAgATcTTTTTTAc at 4 length 25 bases, Tm 54 Reverse TTcAgcTTTTTTTATgggggTT at 131 length 22 bases, Tm 62
SSR size	149 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC239
Source Species	Triticum aestivum