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GrainGenes Probe Report: WMC234

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC234
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5c1 Donor: Sharp, Camden Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts (2) in this clone joined at 349-366 by linkers. First insert matches 7g4. (CA)13 502 to 527. Primers are for second insert.
Type	PCR SSR
PCR primers	Forward cAAggAcTcAATcccTgcATAc at 380 length 22 bases, Tm 63 Reverse AcTgcTTcTTTgTccTccTggT at 572 length 22 bases, Tm 63
SSR size	214 bases expected, Chinese Spring
Sequence	WMC234
Source Species	Triticum aestivum