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GrainGenes Probe Report: WMC232

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC232  [ Marker Report ]
Locus	Xwmc232-4A [ Show all 6 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 5b1.1 Donor: Sharp, Camden Trimmed to Rsa1 at both ends. Multiple inserts (2) joined by linker related sequence 310-317.(CA)11 367 to 388, (CA)14 391 to 418, (GT)23 56 to 101. Primers shown are for first insert.
Type	PCR SSR
PCR primers	Forward gAgATTTgTTcATTTcATcTTcgcA at 23 length 25 bases, Tm 65 Reverse TATATTAAAggTTAgAggTAgTcAg at 106 length 25 bases, Tm 52
SSR size	108 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product;
Sequence	WMC232
Source Species	Triticum aestivum