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GrainGenes Probe Report: WMC231

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC231  [ Marker Report ]
Locus	Xwmc231-3B Xwmc231 wmc231
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11c12 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 140 bases located on 3B, 228 bases located on 3B AGR205.43. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)10 127 to 146, (GT)8 81 to 96.
Type	PCR SSR
PCR primers	Forward cATggcgAggAgcTcggTggTc at 8 length 22 bases, Tm 74 Reverse gTggAgcAcAggcggAgcAAgg at 215 length 22 bases, Tm 74
SSR size	229 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC231
Source Species	Triticum aestivum