Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC230

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC230  [ Marker Report ]
Locus	Xwmc230-1B Xwmc230 wmc230 wmc230-1B
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b12 Donor: Benoist AGR205.42. Apparently no overlap between forward and reverse sequences. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts in this clone joined at 345-369 by linkers. (GT)14 92 to 119. Bases 375-445 match part of 3f10. Bases 459 to 481 show homology to 24c3 bases 274 to 296 (23 in longest run, maxScore = 69).
Type	PCR SSR
PCR primers	Forward AgAAgcgAgcAggTgTgTTTgA at 6 length 22 bases, Tm 66 Reverse cTgcTTccTcccAcAAcAgATg at 195 length 22 bases, Tm 66
SSR size	213 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC230
Source Species	Triticum aestivum