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GrainGenes Probe Report: WMC229

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC229
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f11 Donor: Benoist AGR205.38. Trimmed to Rsa 1 at 5' end and to insert at 3' end. The 3' linker was truncated. (CA)15 209 to 238.
Type	PCR SSR
PCR primers	Forward cggATcATcAgcAAggcAAgTA at 45 length 22 bases, Tm 66 Reverse TgTAAggcAAgTggAcAAgTgg at 285 length 22 bases, Tm 64
SSR size	262 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC229
Source Species	Triticum aestivum