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GrainGenes Probe Report: WMC225

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC225
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g10.1 Donor: Benoist AGR205.31. Trimmed to Rsa1 at both ends. Multiple inserts in this clone joined at 261-278 by linkers. (CA)13 109 to 134, (GT)25 405 to 454. Primers are from first insert. Bases 378 to 475 show homology to 24a3 bases 166 to 263 (complement) (98 in longest run, maxScore = 86).
Type	PCR SSR
PCR primers	Forward cATccTTcAAcTTTTTggTcgg at 12 length 22 bases, Tm 64 Reverse TTATAgAAgcAggcATcTggTg at 159 length 22 bases, Tm 61
SSR size	169 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC225
Source Species	Triticum aestivum