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GrainGenes Probe Report: WMC224

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC224
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f10 Donor: Benoist AGR205.30. No overlap between forward and reverse sequences. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiple inserts (2) in this clone joined at 439-454 by linkers. (GT)50 119 to 218. Primers are from first insert. Bases 470-587 (complement, second insert) match part of 7F11. Bases 481 to 508 show homology to 21a4 bases 40 to 67 (28 in longest run, maxScore = 53).
Type	PCR SSR
PCR primers	Forward gAcAATggAAgccTTgcAgcgg at 24 length 22 bases, Tm 72 Reverse gATggcATggTgAcggTggTgA at 336 length 22 bases, Tm 72
SSR size	334 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product;
Sequence	WMC224
Source Species	Triticum aestivum