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GrainGenes Probe Report: WMC223

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC223  [ Marker Report ]
Locus	Xwmc223
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11e10 Donor: Benoist AGR205.29. Trimmed to insert at both ends. (CA)15 214 to 243.
Type	PCR SSR
PCR primers	Forward cTcTcggTTgcAAAcAAccAcA at 133 length 22 bases, Tm 67 Reverse cAgggAcAAcAcAcgAcAAAgT at 254 length 22 bases, Tm 64
SSR size	143 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC223
Source Species	Triticum aestivum