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GrainGenes Probe Report: WMC221

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC221  [ Marker Report ]
Locus	Xwmc221-7D Xwmc221
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11g9 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 213 bases located on 7D AGR205.23. Trimmed to Rsa1 at both ends. (CA)16 176 to 207, (CT)9 158 to 175.
Type	PCR SSR
PCR primers	Forward AcgATAATgcAgcggggAAT at 40 length 20 bases, Tm 65 Reverse gcTgggATcAAgggATcAAT at 229 length 20 bases, Tm 63
SSR size	209 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC221
Source Species	Triticum aestivum