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GrainGenes Probe Report: WMC220

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC220
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11d9 Donor: Benoist AGR205.20. Trimmed to Rsa1 at 5' end and to insert at 3' end. (CA)10 52 to 71. Bases 98-198 (ie 3' side of microsatellite) match part of 2h12.
Type	PCR SSR
PCR primers	Forward TAcccgcgTcATTTccAcAA at 2 length 20 bases, Tm 66 Reverse TggTTTcgAgcgAgggAgAg at 108 length 20 bases, Tm 67
SSR size	126 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, product;
Sequence	WMC220
Source Species	Triticum aestivum