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GrainGenes Probe Report: WMC219

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC219  [ Marker Report ]
Locus	Xwmc219-4A [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium. MAS Wheat. Bringing Genomics to the Wheat Fields. Disease Resistance. Stripe Rust Resistance. Yr60 MAS Wheat. Marker Assisted Selection in Wheat.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11b9 Donor: Benoist AGR205.18. Overlap of forward and reverse is hypothetical - both end within a substantial micorsatellite. The fragment sizes for the PCR from this sequence will not match the expected size. Trimmed to Rsa1 at both ends. (CA)57 316 to 429.
Type	PCR SSR
PCR primers	Forward TgcTAgTTTgTcATccgggcgA at 264 length 22 bases, Tm 71 Reverse cAATcccgTTcTAcAAgTTccA at 446 length 22 bases, Tm 63
SSR size	~204 bases expected, Chinese Spring
Amplification Conditions	61C anneal, polymorphic product on agarose; 61C anneal, polymorphic on acrylamide;
Sequence	WMC219
Source Gene	Yr60 (Triticum)
Source Species	Triticum aestivum