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GrainGenes Probe Report: WMC218

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC218  [ Marker Report ]
Locus	Xwmc218 Xwmc218-7B wmc218
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11f8 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 105 bases located on 7B AGR205.14. Trimmed to Rsa1 at 5' end and to insert at 3' end. Multiples inserts (3) in this clone joined at 304-321, 502-519 by linkers. Matches 11g8. (TG)7CGTGC(GT)7 microsatellite in first insert. Primers are designed to first insert. Bases 401 to 434 show homology to 22g9 bases 109 to 142 (34 in longest run, maxScore = 52). Bases 28 to 55 show homology to 24b5 bases 153 to 180 (28 in longest run, maxScore = 38).
Type	PCR SSR
PCR primers	Forward TcTccTgTcggcTgAAAgTgTT at 66 length 22 bases, Tm 65 Reverse ccATggAggTTcAccTAgcAAA at 148 length 22 bases, Tm 65
SSR size	104 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC218
Source Species	Triticum aestivum