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GrainGenes Probe Report: WMC217

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC217
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11e8 Donor: Benoist AGR205.13. No overlap between forward and reverse sequences. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)13 151 to 176.
Type	PCR SSR
PCR primers	Forward ATcTcAcTgAAcATAgcTAAAg at 130 length 22 bases, Tm 52 Reverse cAAAAgTAgcTcTcAgcTAAAg at 193 length 22 bases, Tm 55
SSR size	>85 bases expected, Chinese Spring
Amplification Conditions	tested 51C anneal, no product;
Sequence	WMC217
Source Species	Triticum aestivum