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GrainGenes Probe Report: WMC216

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC216  [ Marker Report ]
Locus	Xwmc216-1D [ Show all 8 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11h7 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 128 bases located on 1D AGR205.8. Trimed to insert at 5' end and to Rsa1 at 3' end. (GT)22 42 to 85.
Type	PCR SSR
PCR primers	Forward AcgTATccAgAcAcTgTggTAA at 13 length 22 bases, Tm 59 Reverse TAATggTggATccATgATAgcc at 114 length 22 bases, Tm 63
SSR size	123 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC216
Source Species	Triticum aestivum