Query (optional)   in Class  All Allele Author Colleague Collection Assembly Gene Gene Class Gene Product Germplasm Image Journal Keyword Library Locus Map Map Data Marker Pathology Polymorphism Probe Protein QTL Rearrangement Reference Sequence Species Trait Trait Study Two Point Data  

---

GrainGenes Probe Report: WMC215

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC215  [ Marker Report ]
Locus	Xwmc215-5D [ Show all 7 ]
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11e7 Donor: Benoist Location from nullisomic/tetrasomic (Daryl Somers, AgCanada, Feb 2001) : 211 bases located on 5D AGR205.5. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GT)29 66 to 12.
Type	PCR SSR
PCR primers	Forward cATgcATggTTgcAAgcAAAAg at 16 length 22 bases, Tm 67 Reverse cATcccggTgcAAcATcTgAAA at 201 length 22 bases, Tm 69
SSR size	207 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 61C anneal, polymorphic on acrylamide;
Sequence	WMC215
Source Species	Triticum aestivum