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GrainGenes Probe Report: WMC214

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC214
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 11d7 Donor: Benoist AGR205.4. Trimmed to Rsa1 at 5' end and to insert at 3' end. (GA)9 233 to 250, (GT)10 261 to 280. Bases 77-167 are related to the 1g1, 6b11 family. Bases 233 to 269 show homology to 25b2 bases 84 to 120 (37 in longest run, maxScore = 27). Bases 106 to 143 show homology to 26a11 bases 39 to 76 (38 in longest run, maxScore = 55). Bases 85 to 142 show homology to 14c7 bases 79 to 136 (58 in longest run, maxScore = 52).
Type	PCR SSR
PCR primers	Forward AAgggTggTAggAAcAAATgcT at 100 length 22 bases, Tm 63 Reverse TAAATTAcATccgccATAgAgg at 289 length 22 bases, Tm 60
SSR size	211 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product; 51C anneal, smear;
Sequence	WMC214
Source Species	Triticum aestivum