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GrainGenes Probe Report: WMC211

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC211
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3g4 Donor: Jack, Plant Breeding International No trimming done to insert. Bases 163-337 (ie 3' side of microsatellite) match part of 6d12. Reverse primer is within a perfectly matched section. (CA)15 135 to 164, (CT)11 113 to 134.
Type	PCR SSR
PCR primers	Forward TcAcAcTATATcggccTcTccA at 54 length 22 bases, Tm 64 Reverse gAAcgTTgcAccTTcTcAccTT at 203 length 22 bases, Tm 64
SSR size	171 bases expected, Chinese Spring
Amplification Conditions	61C anneal, product.
Sequence	WMC211
Source Species	Triticum aestivum