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GrainGenes Probe Report: WMC210

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC210
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e4 Donor: Jack, Plant Breeding International No overlap between forward and reverse sequences. No trimming done to insert. Complex GAA and GA microsatellite. Bases 881 to 904 show homology to 10f8 bases 196 to 219 (complement) (24 in longest run, maxScore = 81). Bases 904 to 912 show homology to 2b5 bases 113 to 121 (complement) (9 in longest run, maxScore = 20).
Type	PCR SSR
PCR primers	Forward gccAAcggccTTTgTTgTTAgT at 765 length 22 bases, Tm 66 Reverse TTAAcggAgcgTTTggATTcTT at 1017 length 22 bases, Tm 64
SSR size	274 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide. (several loci).
Sequence	WMC210
Source Species	Triticum aestivum