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GrainGenes Probe Report: WMC208

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC208
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3e3 Donor: Jack, Plant Breeding International unilever comments '(estimated size = 600bp)' No overlap between forward and reverse sequences. No trimming done to insert. (GA)8 825 to 840, (GT)27 285 to 338. Bases 837 to 892 show homology to 26d3 bases 66 to 121 (complement) (56 in longest run, maxScore = 47).
Type	PCR SSR
PCR primers	Forward ATTgccTTTggAcTTccTcAcc at 672 length 22 bases, Tm 65 Reverse cAcAAgcTTgcTTcTTcTTcAT at 845 length 22 bases, Tm 60
SSR size	186 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, product; 51C anneal, polymorphic on acrylamide. (several loci).
Sequence	WMC208
Source Species	Triticum aestivum