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GrainGenes Probe Report: WMC204

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC204
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 3A1 Donor: Jack, Plant Breeding International No trimming done to sequence. (GA)9 811 to 828, (GA)21 831 to 872. Matches 5a2, part match to 4c1. NB this sequence contains numerous ambiguities and a few differences to 5A2, if PCR fails, try designing primers to 5A2. Bases 668 to 732 show homology to 10h10 bases 127 to 191 (complement) (65 in longest run, maxScore = 124). Bases 668 to 740 show homology to 24h2 bases 67 to 139 (73 in longest run, maxScore = 126), true duplicate, 3a1 has priority.
Type	PCR SSR
PCR primers	Forward cATAcATcAAgTgTTcTcAAATcc at 641 length 24 bases, Tm 59 Reverse ccAgTAgcTATgTgTTTgATcTcT at 876 length 24 bases, Tm 58
SSR size	258 bases expected, Chinese Spring
Amplification Conditions	51C anneal, product.
Sequence	WMC204
Source Species	Triticum aestivum