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GrainGenes Probe Report: WMC203

[Submit comment/correction][What is a probe?]Probes originated as records of the single stranded molecules used in RFLP mapping. This term has expanded to include PCR primers and SNPs.

Note: Probe report page includes probe information, gene models, PCR Primers, and SNPs.

Probe	WMC203
Reference	Somers DJ and Isaac P (2004) SSRs from the Wheat Microsatellite Consortium.
General Remarks	Wheat Microsatellite Consortium Project 1, Plate reference 4h12 Donor: Morell, Canberra Trimmed to Rsa1 at 5' end and to insert at 3' end (removing mostly Ns). (GT)10 67 to 86, (GT)34 289 to 356.
Type	PCR SSR
PCR primers	Forward cTAcTTgcATgTcgAATAcccg at 96 length 22 bases, Tm 63 Reverse ccATTcgTggATTTgTTTcAgg at 358 length 22 bases, Tm 65
SSR size	284 bases expected, Chinese Spring
Amplification Conditions	tested 61C anneal, no product; 51C anneal, polymorphic product on agarose; 51C anneal, polymorphic on acrylamide.;
Sequence	WMC203
Source Species	Triticum aestivum